Laser-Activated Fluorescence: A Dynamic Color Change Demonstration (DEMO)

Partha Pratim Mondal | Mondal Lab

"A novel microscopy technique called scanning single molecule localization microscopy (scanSMLM), which significantly enhances super-resolution volume imaging capabilities. This method improves upon traditional single molecule localization microscopy (SMLM) by employing a scanning-based approach for volume imaging, using an electrically tunable lens (ETL) for rapid axial scanning. This enables detailed visualization and analysis of cellular structures, such as actin filaments and mitochondrial networks, and the clustering of Hemagglutinin (HA) protein molecules in cells. The technique's ability to provide three-dimensional imaging at the single-molecule level offers groundbreaking insights into cellular biology and disease progression.."